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> home > Products > Primary Antibodies > Antibody Collections > Mitochondrion > AIFM2 Antibody (C-term)
AIFM2 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | Q9BRQ8 |
| Other Accession | Q8BUE4, A5PJM4 |
| Reactivity | Human |
| Predicted | Bovine, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 40527 Da |
| Antigen Region | 319-348 aa |
| Gene ID | 84883 |
|---|---|
| Other Names | Apoptosis-inducing factor 2, 1---, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, Apoptosis-inducing factor-like mitochondrion-associated inducer of death, p53-responsive gene 3 protein, AIFM2, AMID, PRG3 {ECO:0000303|PubMed:12135761} |
| Target/Specificity | This AIFM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-348 amino acids from the C-terminal region of human AIFM2. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | AIFM2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | AIFM2 {ECO:0000303|PubMed:26689472, ECO:0000312|HGNC:HGNC:21411} |
|---|---|
| Function | A NAD(P)H-dependent oxidoreductase that acts as a key inhibitor of ferroptosis (PubMed:31634899, PubMed:31634900, PubMed:35922516). At the plasma membrane, catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis (PubMed:31634899, PubMed:31634900). Acts in parallel to GPX4 to suppress phospholipid peroxidation and ferroptosis (PubMed:31634899, PubMed:31634900). This anti-ferroptotic function is independent of cellular glutathione levels (PubMed:31634899, PubMed:31634900). Also acts as a potent radical-trapping antioxidant by mediating warfarin-resistant vitamin K reduction in the canonical vitamin K cycle: catalyzes NAD(P)H-dependent reduction of vitamin K (phylloquinone, menaquinone-4 and menadione) to hydroquinone forms (PubMed:35922516). Hydroquinones act as potent radical-trapping antioxidants inhibitor of phospholipid peroxidation and ferroptosis (PubMed:35922516). May play a role in mitochondrial stress signaling (PubMed:26689472). Upon oxidative stress, associates with the lipid peroxidation end product 4-hydroxy-2-nonenal (HNE) forming a lipid adduct devoid of oxidoreductase activity, which then translocates from mitochondria into the nucleus triggering DNA damage and cell death (PubMed:26689472). Capable of DNA binding in a non-sequence specific way (PubMed:15958387). |
| Cellular Location | Lipid droplet. Cell membrane; Lipid-anchor Cytoplasm. Mitochondrion membrane. Nucleus |
| Tissue Location | Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in heart, moderately in liver and skeletal muscles, and expressed at low levels in placenta, lung, kidney, and pancreas. Both transcripts expressed following p53/TP53 induction. The shorter 1.8 kb transcript seems to be the major transcript in EB1 colon cancer cells |
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
AIFM2 is significant homology to NADH oxidoreductases and the apoptosis-inducing factor PDCD8/AIF. The protein has been shown to induce apoptosis. This protein is found to be induced by tumor suppressor protein p53 in colon caner cells.
References
Ohiro Y., Garkavtsev I.FEBS Lett. 524:163-171(2002)
Wu M., Xu L.-G., Su T.Oncogene 23:6815-6819(2004)
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$ 182.50
$ 70.00
Cat# AP1355b
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