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HNMT Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - HNMT Antibody (N-term) AP1442A
    Confocal immunofluorescent analysis of HNMT Antibody (N-term)(Cat#AP1442a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - HNMT Antibody (N-term) AP1442A
    Western blot analysis of HNMT Antibody (N-term) (Cat# AP1442a) in Hela cell line lysates (35ug/lane). HNMT(arrow) was detected using the purified polyclonal antibody.
    detail
  • IHC-P - HNMT Antibody (N-term) AP1442A
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HNMT antibody (N-term) (Cat.#AP1442a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, IF, E
Primary Accession P50135
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 33295 Da
Antigen Region 29-58 aa
Additional Information
Gene ID 3176
Other Names Histamine N-methyltransferase, HMT, HNMT
Target/Specificity This HNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human HNMT.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHNMT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HNMT
Function Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
citation

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Background

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. HNMT is the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells.

References

Aksoy, S., et al., Biochem. Biophys. Res. Commun. 219(2):548-554 (1996).

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$ 182.50
$ 70.00
Cat# AP1442A
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