MINPP1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, WB, E |
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Primary Accession | Q9UNW1 |
Other Accession | NP_004888.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 55051 Da |
Antigen Region | 370-398 aa |
Gene ID | 9562 |
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Other Names | Multiple inositol polyphosphate phosphatase 1, 3-bisphosphoglycerate 3-phosphatase, 3-BPG phosphatase, Inositol (1, 5)-tetrakisphosphate 3-phosphatase, Ins(1, 5)P(4) 3-phosphatase, MINPP1, MIPP |
Target/Specificity | This MINPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 370-398 amino acids from the C-terminal region of human MINPP1. |
Dilution | WB~~1:1000 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | MINPP1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MINPP1 (HGNC:7102) |
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Function | Multiple inositol polyphosphate phosphatase that hydrolyzes 1D-myo-inositol 1,3,4,5,6-pentakisphosphate (InsP5[2OH]) and 1D-myo- inositol hexakisphosphate (InsP6) to a range of less phosphorylated inositol phosphates. This regulates the availability of these various small molecule second messengers and metal chelators which control many aspects of cell physiology (PubMed:36589890, PubMed:33257696). Has a weak in vitro activity towards 1D-myo-inositol 1,4,5-trisphosphate which is unlikely to be physiologically relevant (PubMed:36589890). By regulating intracellular inositol polyphosphates pools, which act as metal chelators, it may control the availability of intracellular calcium and iron, which are important for proper neuronal development and homeostasis (PubMed:33257696). May have a dual substrate specificity, and function as a 2,3-bisphosphoglycerate 3-phosphatase hydrolyzing 2,3-bisphosphoglycerate to 2-phosphoglycerate. 2,3- bisphosphoglycerate (BPG) is formed as part of the Rapoport-Luebering glycolytic bypass and is a regulator of systemic oxygen homeostasis as the major allosteric effector of hemoglobin (PubMed:18413611). |
Cellular Location | Endoplasmic reticulum lumen {ECO:0000250|UniProtKB:O35217}. Secreted Cell membrane {ECO:0000250|UniProtKB:Q9Z2L6}. Note=Also associated with the plasma membrane in erythrocytes. {ECO:0000250|UniProtKB:Q9Z2L6} |
Tissue Location | Widely expressed with highest levels in kidney, liver, cerebellum and placenta. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes multiple inositol polyphosphate phosphatase; an enzyme that removes 3-phosphate from inositol phosphate substrates. It is the only enzyme known to hydrolzye inositol pentakisphosphate and inositol hexakisphosphate. This enzyme also converts 2,3 bisphosphoglycerate (2,3-BPG) to 2-phosphoglycerate; an activity formerly thought to be exclusive to 2,3-BPG synthase/2-phosphatase (BPGM) in the Rapoport-Luebering shunt of the glycolytic pathway.
References
Newman, A.B., et al. J. Gerontol. A Biol. Sci. Med. Sci. 65(5):478-487(2010)
Cho, J., et al. Proc. Natl. Acad. Sci. U.S.A. 105(16):5998-6003(2008)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
Liu, T., et al. J. Proteome Res. 4(6):2070-2080(2005)
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