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COBRA1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q8WX92 |
| Other Accession | Q8C4Y3, NP_056271.2 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 65697 Da |
| Antigen Region | 256-285 aa |
| Gene ID | 25920 |
|---|---|
| Other Names | Negative elongation factor B, NELF-B, Cofactor of BRCA1, NELFB, COBRA1, KIAA1182 |
| Target/Specificity | This COBRA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-285 amino acids from the Central region of human COBRA1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | COBRA1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NELFB |
|---|---|
| Synonyms | COBRA1 {ECO:0000303|PubMed:11739404}, KI |
| Function | Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:12612062). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed:10199401). May be able to induce chromatin unfolding (PubMed:11739404). Essential for early embryogenesis; plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) by preventing unscheduled expression of developmental genes (By similarity). Plays a key role in establishing the responsiveness of stem cells to developmental cues; facilitates plasticity and cell fate commitment in ESCs by establishing the appropriate expression level of signaling molecules (By similarity). Supports the transcription of genes involved in energy metabolism in cardiomyocytes; facilitates the association of transcription initiation factors with the promoters of the metabolism- related genes (By similarity). |
| Cellular Location | Nucleus. |
| Tissue Location | Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).
References
Sun, J., et al. J. Cell. Biochem. 103(6):1798-1807(2008)
Sun, J., et al. J. Steroid Biochem. Mol. Biol. 107 (3-5), 131-139 (2007) :
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Narita, T., et al. Mol. Cell 26(3):349-365(2007)
Aiyar, S.E., et al. Int. J. Biol. Sci. 3(7):486-492(2007)
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