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CDH3 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CDH3 Antibody (C-term) AP1499B
    Anti-CDH3 Antibody (C-term) at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 91 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - CDH3 Antibody (C-term) AP1499B
    Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with CDH3 antibody (C-term) (Cat.#AP1499b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P22223
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 91418 Da
Antigen Region 660-688 aa
Additional Information
Gene ID 1001
Other Names Cadherin-3, Placental cadherin, P-cadherin, CDH3, CDHP
Target/Specificity This CDH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 660-688 amino acids from the C-terminal region of human CDH3.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDH3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDH3
Synonyms CDHP
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Expressed in some normal epithelial tissues and in some carcinoma cell lines.
Research Areas
Citations (0)
citation

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Background

CDH3 is a classical cadherin from the cadherin superfamily. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in the gene encoding CDH3 have been associated with congential hypotrichosis with juvenile macular dystrophy.

References

Indelman,M., Clin. Exp. Dermatol. 32 (2), 191-196 (2007)
Paredes,J., Clin. Cancer Res. 11 (16), 5869-5877 (2005)

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$ 365.00
$ 140.00
Cat# AP1499B
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