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BARON Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - BARON Antibody (Center) AP1831c
    All lanes : Anti-BARON Antibody (Center) at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 109 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - BARON Antibody (Center) AP1831c
    Formalin-fixed and paraffin-embedded human kidney reacted with BARON Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - BARON Antibody (Center) AP1831c
    BARON Antibody (Center) (Cat. #AP1831c) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q92622
Other Accession Q80U62
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 108622 Da
Antigen Region 656-684 aa
Additional Information
Gene ID 9711
Other Names Run domain Beclin-1 interacting and cysteine-rich containing protein, Rubicon, Beclin-1 associated RUN domain containing protein, Baron, KIAA0226
Target/Specificity This BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656-684 amino acids from the Central region of human BARON.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBARON Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RUBCN (HGNC:28991)
Synonyms KIAA0226
Function Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation (PubMed:20974968, PubMed:21062745).
Cellular Location Late endosome. Lysosome. Early endosome Note=Predominantly located in late endosomes/lysosomes, only partially detected in early endosome and not at all in the Golgi apparatus
Research Areas
Citations (0)
citation

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References

Matsunaga, K., et al. Nat. Cell Biol. 11(4):385-396(2009)
Muzny, D.M., et al. Nature 440(7088):1194-1198(2006)
Venter, J.C., et al. Science 291(5507):1304-1351(2001)

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$ 365.00
$ 140.00
Cat# AP1831c
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