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ACTR3 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ACTR3 Antibody (C-term) AP2891b
    Western blot analysis of ACTR3 Antibody (C-term) (Cat. #AP2891b) in A2058 cell line lysates (35ug/lane). ACTR3 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - ACTR3 Antibody (C-term) AP2891b
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ACTR3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - ACTR3 Antibody (C-term) AP2891b
    Flow cytometric analysis of hela cells using ACTR3 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P61158
Other Accession Q4V7C7, Q99JY9, Q90WD0, P61157, Q801P7, A0A1L8EV45
Reactivity Human
Predicted Xenopus, Bovine, Chicken, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 47371 Da
Antigen Region 380-407 aa
Additional Information
Gene ID 10096
Other Names Actin-related protein 3, Actin-like protein 3, ACTR3, ARP3
Target/Specificity This ACTR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-407 amino acids from the C-terminal region of human ACTR3.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACTR3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTR3
Synonyms ARP3
Function ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9000076). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9000076). Seems to contact the pointed end of the daughter actin filament (PubMed:9000076). In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation (PubMed:29058690). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:17220302, PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947). Plays a role in ciliogenesis (PubMed:20393563).
Cellular Location Cytoplasm, cytoskeleton. Cell projection. Nucleus. Note=In pre- apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes) (PubMed:19109554)
Research Areas
Citations (0)
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Background

ACTR3 is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.

References

Weisswange,I., et. al., Nature 458 (7234), 87-91 (2009)

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$ 182.50
$ 70.00
Cat# AP2891b
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