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EGR1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - EGR1 Antibody (N-term) AP2904a
    Confocal immunofluorescent analysis of EGR1 Antibody (N-term)(Cat#AP2904a) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - EGR1 Antibody (N-term) AP2904a
    Western blot analysis of EGR1 Antibody (N-term) (Cat. #AP2904a) in mouse NIH-3T3 tissue lysates (35ug/lane). EGR1 (arrow) was detected using the purified Pab.
    detail
  • WB - EGR1 Antibody (N-term) AP2904a
    EGR1 Antibody (N-term) (Cat. #AP2904a) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the EGR1 antibody detected the EGR1 protein (arrow).
    detail
  • FC - EGR1 Antibody (N-term) AP2904a
    EGR1 Antibody (N-term) (Cat. #AP2904a) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB, FC, E
Primary Accession P18146
Other Accession P08046, Q29W20, Q6GQH4
Reactivity Human, Mouse
Predicted Xenopus, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 57507 Da
Antigen Region 9-37 aa
Additional Information
Gene ID 1958
Other Names Early growth response protein 1, EGR-1, AT225, Nerve growth factor-induced protein A, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225, Zinc finger protein Krox-24, EGR1, KROX24, ZNF225
Target/Specificity This EGR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of human EGR1.
Dilution IF~~1:10~50
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEGR1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EGR1
Synonyms KROX24, ZNF225 {ECO:0000303|PubMed:21103
Function Transcriptional regulator (PubMed:20121949). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes (By similarity). Binds double-stranded target DNA, irrespective of the cytosine methylation status (PubMed:25258363, PubMed:25999311). Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary (By similarity). Regulates the amplitude of the expression rhythms of clock genes: BMAL1, PER2 and NR1D1 in the liver via the activation of PER1 (clock repressor) transcription. Regulates the rhythmic expression of core-clock gene BMAL1 in the suprachiasmatic nucleus (SCN) (By similarity).
Cellular Location Nucleus. Cytoplasm
Tissue Location Detected in neutrophils (at protein level).
Research Areas
Citations (0)
citation

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Background

EGR1 belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis.

References

Chan,I.H., et.al., Clin. Chim. Acta 411 (1-2), 67-71 (2010)

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$ 182.50
$ 70.00
Cat# AP2904a
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