|Application ||IHC-P, WB, E|
|Calculated MW||62720 Da|
|Other Names||Cell division control protein 6 homolog, CDC6-related protein, Cdc18-related protein, HsCdc18, p62(cdc6), HsCDC6, CDC6, CDC18L|
|Target/Specificity||This CDC6 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S54 of human CDC6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-CDC6(S54) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.|
|Cellular Location||Nucleus. Cytoplasm. Note=The protein is nuclear in G1 and cytoplasmic in S-phase cells (PubMed:9566895)|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.
Alexandrow, M.G., et al., Mol. Cell. Biol. 24(4):1614-1627 (2004).
Yim, H., et al., Mol. Biol. Cell 14(10):4250-4259 (2003).
Clay-Farrace, L., et al., EMBO J. 22(3):704-712 (2003).
Pelizon, C., et al., EMBO Rep. 3(8):780-784 (2002).
Bermejo, R., et al., Mol. Biol. Cell 13(11):3989-4000 (2002).
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