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CYB5R3 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CYB5R3 Antibody AP50643
    Western blot analysis of lysates from A431,U-87 MG cell line (from left to right),using CYB5R3 Antibody(AP50643). AP50643 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
    detail
  • IHC - CYB5R3 Antibody AP50643
    Immunohistochemistry analysis of paraffin-embedded human placenta tissue using CYB5R3 antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P00387
Host Rabbit
Clonality Polyclonal
Calculated MW 34 32 38 KDa
Antigen Region 148-175 aa
Additional Information
Gene ID 1727
Other Names NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, Diaphorase-1, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, CYB5R3, DIA1
Dilution WB~~1:1000
IHC~~1:50-1:100
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.09% (W/V) sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name CYB5R3 (HGNC:2873)
Synonyms DIA1
Function Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
Cellular Location [Isoform 1]: Endoplasmic reticulum membrane; Lipid-anchor {ECO:0000250|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250|UniProtKB:P20070}. Mitochondrion outer membrane; Lipid-anchor {ECO:0000250|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250|UniProtKB:P20070}
Tissue Location [Isoform 2]: Expressed at late stages of erythroid maturation.
Research Areas
Citations (0)
citation

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Background

Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

References

Tomatsu S.,et al.Gene 80:353-361(1989).
Voice M.W.,et al.Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.
Yoon B.,et al.Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
Lan F.,et al.Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.

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$ 350.00
Cat# AP50643
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