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Anti-β-Actin Antibody

Mouse Anti Human Monoclonal Antibody

     
  • WB - Anti-β-Actin Antibody AP53385
    Western blot detection of beta actin in Jurkat,K562,C6,3T3 and Hela cell lysates using beta actin mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
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  • SPECIFICATION
  • CITATIONS: 5
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P60709
Other Accession NM_001101
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Immunogen Purified recombinant human beta actin protein fragments expressed in E.coli.
Purification Affinity purified
Calculated MW 45 KDa
Additional Information
Gene ID 60
Other Names A X actin like protein;ACTB;ACTB_HUMAN;Actin;Actin cytoplasmic 1;Actx;b actin;b-actin;Beta cytoskeletal actin;Beta-actin;cytoplasmic 1;Melanoma X actin;N-terminally processed;PS1TP5 binding protein 1;PS1TP5BP1.
Dilution WB~~1:1000
Format Purified mouse monoclonal antibody in PB(pH 7.4) containing with 0.09% (W/V) sodium azide,0.1mg/mlBSA and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ACTB
Function Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Cellular Location Cytoplasm, cytoskeleton. Nucleus Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Research Areas
Citations ( 0 )

Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

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$ 370.00
Cat# AP53385
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