EEF1D Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, IHC-P, WB, E |
---|---|
Primary Accession | P29692 |
Other Accession | Q68FR9, P57776, Q4R3D4 |
Reactivity | Human |
Predicted | Monkey, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 31122 Da |
Antigen Region | 1-30 aa |
Gene ID | 1936 |
---|---|
Other Names | Elongation factor 1-delta, EF-1-delta, Antigen NY-CO-4, EEF1D, EF1D |
Target/Specificity | This EEF1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EEF1D. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | EEF1D Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | EEF1D |
---|---|
Synonyms | EF1D |
Function | [Isoform 1]: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome. |
Cellular Location | [Isoform 2]: Nucleus |
Tissue Location | Isoform 2 is specifically expressed in brain, cerebellum and testis |
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Provided below are standard protocols that you may find useful for product applications.
Background
EEF1D is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs.
References
Yang,S., BMC Cancer 7, 211 (2007)
Mulner-Lorillon,O., J. Biol. Chem. 269 (31), 20201-20207 (1994)
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