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EMILIN1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - EMILIN1 Antibody (N-term) AP6640a
    Western blot analysis of EMILIN1 Antibody (N-term) (Cat. #AP6640a) in K562 and 293 cell line lysates (35ug/lane). EMILIN1 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - EMILIN1 Antibody (N-term) AP6640a
    Formalin-fixed and paraffin-embedded human skeletal muscle with EMILIN1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - EMILIN1 Antibody (N-term) AP6640a
    Flow cytometric analysis of 293 cells using EMILIN1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q9Y6C2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 106695 Da
Antigen Region 84-112 aa
Additional Information
Gene ID 11117
Other Names EMILIN-1, Elastin microfibril interface-located protein 1, Elastin microfibril interfacer 1, EMILIN1, EMI
Target/Specificity This EMILIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-112 amino acids from the N-terminal region of human EMILIN1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEMILIN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EMILIN1
Synonyms EMI
Function May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity.
Cellular Location Secreted, extracellular space, extracellular matrix Note=Found mainly at the interface between amorphous elastin and microfibrils.
Tissue Location Distributed in tissues where resilience and elastic recoil are prominent. Highest levels in the adult small intestine, aorta, lung, uterus, and appendix and in the fetal spleen, kidney, lung, and heart; intermediate expression was detected in adult liver, ovary, colon, stomach, lymph node and spleen; adult heart, bladder, prostate, adrenal gland, mammary gland, placenta and kidney showed low expression whereas a series of other adult tissues, including skeletal muscle and different regions of adult brain show no expression Detected in intramuscular nerve bundles, where it particularly localizes in the epineurium, the most external layer of dense connective tissue enclosing the nerve (PubMed:31978608)
Research Areas
Citations (0)
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Background

EMILIN1 may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity.

References

Verdone,G., J. Biol. Chem. 283 (27), 18947-18956 (2008)
Danussi,C., Mol. Cell. Biol. 28 (12), 4026-4039 (2008)

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$ 365.00
$ 140.00
Cat# AP6640a
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