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HIPK4 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HIPK4 Antibody (C-term) AP7138b
    Western blot analysis of HIPK4 C-term (Cat. #AP7138b) in HL-60 cell line lysates (35ug/lane). HIPK4 (arrow) was detected using the purified Pab.
    detail
  • WB - HIPK4 Antibody (C-term) AP7138b
    Western blot analysis of HIPK4 (arrow) using rabbit polyclonal HIPK4 Antibody (C-term) (Cat. #AP7138b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HIPK4 gene.
    detail
  • WB - HIPK4 Antibody (C-term) AP7138b
    Anti-HIPK4 C-term at 1:1000 dilution + H. testis whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8NE63
Other Accession Q8WP28
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 69425 Da
Antigen Region 537-566 aa
Additional Information
Gene ID 147746
Other Names Homeodomain-interacting protein kinase 4, HIPK4
Target/Specificity This HIPK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 537-566 amino acids from the C-terminal region of human HIPK4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIPK4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HIPK4
Function Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors (Potential).
Cellular Location Cytoplasm.
Research Areas
Citations ( 0 )

Background

HIPK4 is a serine/threonine protein kinase that may act as a corepressor of transcription factors.

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$ 182.50
$ 70.00
Cat# AP7138b
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