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PDXK Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | O00764 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 35102 Da |
| Antigen Region | 242-272 aa |
| Gene ID | 8566 |
|---|---|
| Other Names | Pyridoxal kinase, Pyridoxine kinase, PDXK, C21orf124, C21orf97, PKH, PNK |
| Target/Specificity | This PDXK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-272 amino acids from the C-terminal region of human PDXK. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PDXK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PDXK (HGNC:8819) |
|---|---|
| Function | Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (PubMed:9099727, PubMed:10987144, PubMed:17766369, PubMed:19351586, PubMed:31187503) (Probable). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions. |
| Cellular Location | Cytoplasm, cytosol. |
| Tissue Location | Ubiquitous (PubMed:9099727, PubMed:31187503). Highly expressed in testis (PubMed:9099727) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Pyridoxal kinase (PDXK) converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in the intermediate metabolism of amino acids and neurotransmitters. The PDXK gene encodes a 312-amino acid polypeptide, and expression of the cDNA reveals pyridoxal kinase activity. Northern blot analysis revealed that a major 1.5-kb PDXK transcript is expressed in all tissues tested. The expression of PDXK shows circadian oscillations. The expression of Pdxk in mouse liver and brain is regulated by the 3 PAR bZIP transcription factors, Dbp, Hlf, and Tef, which also show circadian oscillations in expression. Mice devoid of all 3 transcription factors show decreased levels of brain PLP, serotonin, and dopamine, and are highly susceptible to frequently lethal generalized spontaneous and audiogenic epilepsies.
References
Shin, J.H., et al., Neurochem. Int. 45(1):73-79 (2004).
Lee, H.S., et al., Mol. Cells 10(4):452-459 (2000).
Laine-Cessac, P., et al., Biochem. Pharmacol. 54(8):863-870 (1997).
Hanna, M.C., et al., J. Biol. Chem. 272(16):10756-10760 (1997).
Zhang, Z., et al., J. Nutr. 10(1):53-59 (1993).
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