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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ILK Antibody (S246)   

ILK Antibody (S246)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ILK Antibody (S246) AP7651e
    Western blot analysis of anti-ILK Antibody (S246) (Cat.#AP7651e) in Hela cell line lysates (35ug/lane). ILK(arrow) was detected using the purified Pab.
    detail
  • WB - ILK Antibody (S246) AP7651e
    Western blot analysis of ILK (arrow) using rabbit polyclonal ILK Antibody (pS246) (Cat.#AP7651e). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ILK gene.
    detail
  • IHC-P - ILK Antibody (S246) AP7651e
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK Antibody (S246) (Cat.#AP7651e), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
  • CITATIONS: 1
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q13418
Other Accession Q99J82, O55222, Q3SWY2, NP_004508
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 51419 Da
Antigen Region 225-253 aa
Additional Information
Gene ID 3611
Other Names Integrin-linked protein kinase, 59 kDa serine/threonine-protein kinase, ILK-1, ILK-2, p59ILK, ILK, ILK1, ILK2
Target/Specificity This ILK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-253 amino acids from human ILK.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsILK Antibody (S246) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ILK (HGNC:6040)
Function Receptor-proximal protein kinase regulating integrin-mediated signal transduction (PubMed:8538749, PubMed:9736715). May act as a mediator of inside-out integrin signaling (PubMed:10712922). Focal adhesion protein part of the complex ILK-PINCH (PubMed:10712922). This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway (PubMed:10712922). Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells (PubMed:10712922). Regulates cell motility by forming a complex with PARVB (PubMed:32528174). Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (PubMed:8538749, PubMed:9736715).
Cellular Location Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium {ECO:0000250|UniProtKB:O55222}. Cytoplasm, myofibril, sarcomere
Tissue Location Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver
Research Areas
Citations ( 0 )

Background

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. ILK is a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction.

References

Li, Y., et al., J. Clin. Invest. 112(4):503-516 (2003).
Troussard, A.A., et al., J. Biol. Chem. 278(25):22374-22378 (2003).
Marotta, A., et al., Br. J. Cancer 88(11):1755-1762 (2003).
Cordes, N., et al., Br. J. Cancer 88(9):1470-1479 (2003).
Fukuda, T., et al., J. Cell Biol. 160(7):1001-1008 (2003).

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$ 182.50
$ 70.00
Cat# AP7651e
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