CYP4X1 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
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Primary Accession | Q8N118 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 58875 Da |
Antigen Region | 157-183 aa |
Gene ID | 260293 |
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Other Names | Cytochrome P450 4X1, CYPIVX1, CYP4X1 |
Target/Specificity | This CYP4X1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-183 amino acids from the N-terminal region of human CYP4X1. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | CYP4X1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CYP4X1 {ECO:0000303|PubMed:18549450, ECO:0000312|HGNC:HGNC:20244} |
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Function | A cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, potentially modulating endocannabinoid signaling. Has no hydroxylase activity toward various fatty acids, steroids and prostaglandins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). |
Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q6A152}; Single-pass membrane protein. Microsome membrane {ECO:0000250|UniProtKB:Q6A152}; Single-pass membrane protein |
Tissue Location | Expressed in brain, heart, kidney and skin and, at lower levels, in skeletal muscle and liver (PubMed:16478468, PubMed:18549450). In the brain, high levels are detected in amygdala and lower levels in globus pallidus and cerebellum (PubMed:18549450) In the heart, very high levels in aorta, but very low levels in other heart regions (PubMed:16478468, PubMed:18549450). Also expressed in breast, prostate and colon (PubMed:18549450) |
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Provided below are standard protocols that you may find useful for product applications.
Background
CYP4X1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The expression pattern of a similar rat protein suggests that this protein may be involved in neurovascular function in the brain.
References
Savas,U., Arch. Biochem. Biophys. 436 (2), 377-385 (2005)
Nelson,D.R., Pharmacogenetics 14 (1), 1-18 (2004)
Bylund,J., Biochem. Biophys. Res. Commun. 296 (3), 677-684 (2002)
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