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CCHCR1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - CCHCR1 Antibody (Center) AP8528C
    Confocal immunofluorescent analysis of CCHCR1 Antibody (Center) (Cat. #AP8528c) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green) .DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - CCHCR1 Antibody (Center) AP8528C
    Anti-CCHCR1 Antibody (Center) at 1:1000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 89 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - CCHCR1 Antibody (Center) AP8528C
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with CCHCR1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - CCHCR1 Antibody (Center) AP8528C
    Flow cytometric analysis of widr cells using CCHCR1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, FC, IHC-P, WB, E
Primary Accession Q8TD31
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 88671 Da
Antigen Region 599-627 aa
Additional Information
Gene ID 54535
Other Names Coiled-coil alpha-helical rod protein 1, Alpha-helical coiled-coil rod protein, Putative gene 8 protein, Pg8, CCHCR1, C6orf18, HCR
Target/Specificity This CCHCR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 599-627 amino acids from the Central region of human CCHCR1.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCHCR1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCHCR1
Synonyms C6orf18, HCR
Function May be a regulator of keratinocyte proliferation or differentiation.
Cellular Location Cytoplasm. Nucleus.
Tissue Location Found in all tissues tested, abundantly expressed in heart, liver, skeletal muscle, kidney and pancreas, and to a lesser extent in lung and placenta. Overexpressed in keratinocytes of psoriatic lesions
Citations (0)
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Background

CCHCR1 may be a regulator of keratinocyte proliferation or differentiation.

References

Asumalahti,K., et.al., Hum. Mol. Genet. 9 (10), 1533-1542 (2000)
Oka,A., et.al., Hum. Mol. Genet. 8 (12), 2165-2170 (1999)

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$ 365.00
$ 140.00
Cat# AP8528C
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