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PTGER3 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PTGER3 Antibody (N-term) AP9132A
    Anti-PTGER3 Antibody (N-term) at 1:500 dilution + Human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - PTGER3 Antibody (N-term) AP9132A
    Western blot analysis of PTGER3 Antibody (N-term) (Cat. #AP9132a) in Ramos cell line lysates (35ug/lane). PTGER3 (arrow) was detected using the purified Pab.
    detail
  • WB - PTGER3 Antibody (N-term) AP9132A
    All lanes : Anti-PTGER3 Antibody (N-term) at 1:1000 dilution Lane 1: Ramos whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: 293 whole cell lysate Lane 4: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - PTGER3 Antibody (N-term) AP9132A
    Overlay histogram showing Ramos cells stained with AP9132A(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P43115
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 43310 Da
Antigen Region 17-46 aa
Additional Information
Gene ID 5733
Other Names Prostaglandin E2 receptor EP3 subtype, PGE receptor EP3 subtype, PGE2 receptor EP3 subtype, PGE2-R, Prostanoid EP3 receptor, PTGER3
Target/Specificity This PTGER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human PTGER3.
Dilution WB~~1:1000
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPTGER3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTGER3
Function Receptor for prostaglandin E2 (PGE2) (PubMed:8307176, PubMed:7883006, PubMed:8117308, PubMed:8135729, PubMed:7981210). The activity of this receptor can couple to both the inhibition of adenylate cyclase mediated by G(i) proteins, and to an elevation of intracellular calcium (PubMed:7883006, PubMed:8117308, PubMed:8135729, PubMed:7981210). Required for normal development of fever in response to pyrinogens, including IL1B, prostaglandin E2 and bacterial lipopolysaccharide (LPS). Required for normal potentiation of platelet aggregation by prostaglandin E2, and thus plays a role in the regulation of blood coagulation. Required for increased HCO3(-) secretion in the duodenum in response to mucosal acidification, and thereby contributes to the protection of the mucosa against acid- induced ulceration. Not required for normal kidney function, normal urine volume and osmolality (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Detected in kidney (PubMed:8117308, PubMed:8135729). Expressed in small intestine, heart, pancreas, gastric fundic mucosa, mammary artery and pulmonary vessels
Research Areas
Citations (0)
citation

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Background

PTGER3 is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor may have many biological functions, which involve digestion, nervous system, kidney reabsorption, and uterine contraction activities. Studies of the mouse counterpart suggest that this receptor may also mediate adrenocorticotropic hormone response as well as fever generation in response to exogenous and endogenous stimuli.

References

Schmid,A., et.al., Eur. J. Biochem. 228 (1), 23-30 (1995)
An,S., et.al., Biochemistry 33 (48), 14496-14502 (1994)

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$ 182.50
$ 70.00
Cat# AP9132A
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