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DHRS3 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DHRS3 Antibody (Center) AP9188c
    DHRS3 Antibody (Center) (Cat. #AP9188c) western blot analysis in HepG2 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the DHRS3 antibody detected the DHRS3 protein (arrow).
    detail
  • WB - DHRS3 Antibody (Center) AP9188c
    Western blot analysis of DHRS3 (arrow) using rabbit polyclonal DHRS3 Antibody (Center) (Cat. #AP9188c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DHRS3 gene.
    detail
  • WB - DHRS3 Antibody (Center) AP9188c
    All lanes : Anti-DHRS3 Antibody (Center) at 1:1000 dilution Lane 1: A375 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O75911
Other Accession O77769
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 33548 Da
Antigen Region 85-113 aa
Additional Information
Gene ID 9249
Other Names Short-chain dehydrogenase/reductase 3, DD831, Retinal short-chain dehydrogenase/reductase 1, retSDR1, DHRS3
Target/Specificity This DHRS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-113 amino acids from the Central region of human DHRS3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDHRS3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DHRS3
Synonyms RDH17, SDR16C1
Function Catalyzes the reduction of all-trans-retinal to all-trans- retinol in the presence of NADPH.
Cellular Location Membrane; Multi-pass membrane protein
Tissue Location Widely expressed with highest levels found in heart, placenta, lung, liver, kidney, pancreas, thyroid, testis, stomach, trachea and spinal cord. Lower levels found in skeletal muscle, intestine and lymph node. No expression detected in brain. In the retina, expressed in cone but not rod outer segments
Research Areas
Citations (0)
citation

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Background

DHRS3 catalyze the oxidation/reduction of a wide range of substrates, including retinoids and steroids.

References

Persson,B., et.al., Chem. Biol. Interact. 178 (1-3), 94-98 (2009)
Roni,V., et.al., BMC Genomics 8, 42 (2007)

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$ 182.50
$ 70.00
Cat# AP9188c
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