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AMY1A Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - AMY1A Antibody (Center) AP9519c
    Western blot analysis of AMY1A Antibody (Center) (Cat. #AP9519c) in HL-60 cell line lysates (35ug/lane). AMY1A (arrow) was detected using the purified Pab.
    detail
  • IHC-P - AMY1A Antibody (Center) AP9519c
    Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with AMY1A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - AMY1A Antibody (Center) AP9519c
    AMY1A Antibody (Center) (Cat. #AP9519c) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P04745
Other Accession P00690, P0DTE7, P0DTE8, P0DUB6
Reactivity Human
Predicted Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Antigen Region 195-223 aa
Additional Information
Other Names Alpha-amylase 1, 4-alpha-D-glucan glucanohydrolase 1, Salivary alpha-amylase, AMY1A, AMY1
Target/Specificity This AMY1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-223 amino acids from the Central region of human AMY1A.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAMY1A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)
citation

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Background

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. It is an amylase isoenzyme produced by the salivary gland.

References

Ragunath, C., et al. J. Mol. Biol. 384(5):1232-1248(2008)
Takahashi, T., et al. Neuro Endocrinol. Lett. 28(5):662-665(2007)
Perry, G.H., et al. Nat. Genet. 39(10):1256-1260(2007)
Chaudhuri, B., et al. BMC Microbiol. 7, 60 (2007) :
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Bank, R.A., et al. Electrophoresis 12(1):74-79(1991)

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$ 365.00
$ 140.00
Cat# AP9519c
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