Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
LDHA Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | P00338 |
| Other Accession | P04642, P00339, Q9BE24, P19858 |
| Reactivity | Human |
| Predicted | Bovine, Monkey, Pig, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 36689 Da |
| Antigen Region | 154-182 aa |
| Gene ID | 3939 |
|---|---|
| Other Names | L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, LDHA |
| Target/Specificity | This LDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-182 amino acids from the Central region of human LDHA. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | LDHA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | LDHA (HGNC:6535) |
|---|---|
| Function | Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+). |
| Cellular Location | Cytoplasm. |
| Tissue Location | Predominantly expressed in anaerobic tissues such as skeletal muscle and liver. |
Provided below are standard protocols that you may find useful for product applications.
Background
LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria.
References
Zhu, X., et al. Genet. Epidemiol. 34(2):171-187(2010)
Zhuang, L., et al. Mod. Pathol. 23(1):45-53(2010)
Zhao, Y.H., et al. Oncogene 28(42):3689-3701(2009)
Xie, H., et al. Mol. Cancer Ther. 8(3):626-635(2009)
Koukourakis, M.I., et al. Oncology 77(5):285-292(2009)
Rikova, K., et al. Cell 131(6):1190-1203(2007)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
