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MAT2B Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MAT2B Antibody (N-term) AP9534a
    Western blot analysis of MAT2B Antibody (N-term) (Cat. #AP9534a) in293,MDA-MB231 cell line lysates (35ug/lane). MAT2B (arrow) was detected using the purified Pab.
    detail
  • IHC-P - MAT2B Antibody (N-term) AP9534a
    Formalin-fixed and paraffin-embedded human breast carcinoma reacted with MAT2B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9NZL9
Other Accession NP_037415
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 37552 Da
Antigen Region 80-107 aa
Additional Information
Gene ID 27430
Other Names Methionine adenosyltransferase 2 subunit beta, Methionine adenosyltransferase II beta, MAT II beta, Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase, MAT2B, TGR
Target/Specificity This MAT2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-107 amino acids from the N-terminal region of human MAT2B.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAT2B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAT2B
Synonyms TGR
Function Regulatory subunit of S-adenosylmethionine synthetase 2, an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates MAT2A catalytic activity by changing its kinetic properties, increasing its affinity for L-methionine (PubMed:10644686, PubMed:23189196, PubMed:25075345). Can bind NADP (in vitro) (PubMed:23189196, PubMed:23425511).
Tissue Location Widely expressed.
Research Areas
Citations (0)
citation

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Background

MAT2B belongs to the methionine adenosyltransferase (MAT) family. MAT catalyzes the biosynthesis of S-adenosylmethionine from methionine and ATP. This protein is the regulatory beta subunit of MAT.

References

Martinez, C.A., et al. Am. J. Obstet. Gynecol. 201 (4), 394 (2009) :
Boyles, A.L., et al. Genet. Epidemiol. 33(3):247-255(2009)
Persson, B., et al. Chem. Biol. Interact. 178 (1-3), 94-98 (2009) :
Attia, R.R., et al. J. Biol. Chem. 283(45):30788-30795(2008)
Wang, Q., et al. World J. Gastroenterol. 14(29):4633-4642(2008)
LeGros, L., et al. J. Biol. Chem. 276(27):24918-24924(2001)

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$ 365.00
$ 140.00
Cat# AP9534a
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