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TWA1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q9NWU2 |
| Other Accession | NP_060366, 8923557 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 26749 Da |
| Application Notes | TWA1 antibody can be used for detection of TWA1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 54994 |
|---|---|
| Target/Specificity | C20orf11; |
| Reconstitution & Storage | TWA1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | TWA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GID8 |
|---|---|
| Function | Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (PubMed:29911972). Acts as a positive regulator of Wnt signaling pathway by promoting beta-catenin (CTNNB1) nuclear accumulation (PubMed:28829046). |
| Cellular Location | Cytoplasm. Nucleus. Note=Localizes in the cytoplasm in the absence of Wnt stimulation and in the nucleus in the presence of Wnt stimulation. |
| Tissue Location | Up-regulated in colorectal cancer tissues (at protein level). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
TWA1 Antibody: TWA1 was identified through a two hybrid-associated protein screen with RanBPM. TWA1 is well conserved through evolution and is localized within the nucleus. It interacts with RanBP9 and comprises a protein complex with RanBPM and Muskelin. TWA1 was found to possess the LisH-CTLH motif which is detected in proteins involved in microtubule dynamics, cell migration, nucleokinesis and chromosome segregation. These functions overlap with functions suggested for the RanGTPase cycle. Recent findings suggested that there is an as yet uncovered function of the RanGTPase cycle.
References
Umeda M, Nishitani H, and Nishimoto T. A novel nuclear protein, Twa1, and Muskelin comprise a complex with RanBPM. Gene2003; 303:47-54.
Kobayashi N, Yang J, Ueda A, et al. RanBPM, Muskelin, p48EMLP, p44CTLH, and the armadillo-repeat proteins ARMC8alpha and ARMC8beta are components of the CTLH complex. Gene2007; 396:236-47.
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