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CCDC55 Antibody

     
  • WB - CCDC55 Antibody ASC11384
    Western blot analysis of CCDC55 in human brain tissue lysate with CCDC55 antibody at (A) 0.5 and (B) 1 µg/mL
    detail
  • IF - CCDC55 Antibody ASC11384
    Immunofluorescence of CCDC55 in mouse brain cells with CCDC55 antibody at 20 µg/mL.
    detail
  • SPECIFICATION
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q9H0G5
Other Accession NP_115517, 14149807
Reactivity Human, Mouse, Rat
Host Chicken
Clonality Polyclonal
Isotype IgY
Calculated MW 66390 Da
Application Notes CCDC55 antibody can be used for detection of CCDC55 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunofluorescence starting at 20 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 84081
Target/Specificity NSRP1; At least three isoforms are known to exist; this antibody will detect all three.
Reconstitution & Storage CCDC55 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsCCDC55 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NSRP1
Synonyms CCDC55, NSRP70
Function RNA-binding protein that mediates pre-mRNA alternative splicing regulation.
Cellular Location Nucleus. Nucleus speckle. Note=Colocalizes with splicing factors SRSF1 and SRSF2 in speckles
Tissue Location Expressed in dendritic cells, T-cells, B-cells and natural killer cells. Expressed in secondary lymphoid organs such as spleen and mesenteric, axillary and brachial lymph nodes
Research Areas
Citations (0)
citation

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Background

CCDC55 Antibody: CCDC55, also known as Nuclear speckle-related protein 70 (NSrp70), was initially identified by the NIH Mammalian Gene Collection. Nuclear speckles are known to be the storage sites of mRNA splicing regulators. CCDC55 has been suggested to modulate alternative pre-mRNA splicing in vivo. It co-localizes and physically interacts with the SR proteins SC35 and ASF/SF2, essential splicing factors which are required for constitutive splicing and can regulate alternative mRNA splicing. Loss of CCDC55 in mice leads to early embryonic lethality highlights the importance of the functional role of CCDC55.

References

Kim YD, Lee JY, Oh KM, et al. NSrp70 is a novel nuclear speckle-related protein that modulates alternative pre-mRNA splicing in vivo. Nuc. Acids Res. 2011; 39:4300-14.


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$ 379.00
Cat# ASC11384
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