DEPTOR Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | Q8TB45 |
Other Accession | NP_073620, 189571664 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | Predicted: 45 kDa Observed: 42 kDa |
Application Notes | DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. |
Gene ID | 64798 |
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Target/Specificity | DEPTOR; DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist; this antibody will detect all three isoforms. |
Reconstitution & Storage | DEPTOR antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
Precautions | DEPTOR Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | DEPTOR {ECO:0000303|PubMed:19446321, ECO:0000312|HGNC:HGNC:22953} |
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Function | Negative regulator of the mTORC1 and mTORC2 complexes: inhibits the protein kinase activity of MTOR, thereby inactivating both complexes (PubMed:19446321, PubMed:22017875, PubMed:22017876, PubMed:22017877, PubMed:25936805, PubMed:29382726, PubMed:34519269, PubMed:34519268). DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy (PubMed:22017875, PubMed:22017876, PubMed:22017877). In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity (PubMed:34519269, PubMed:34519268). |
Cellular Location | Lysosome membrane. Note=Localizes to the lysosomal membrane when associated with the mTORC1 and mTORC2 complexes. |
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Provided below are standard protocols that you may find useful for product applications.
Background
DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).
References
Peterson TR, Laplante M, Thoreen CC, et al. DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for survival. Cell 2009; 137:873-6.
Duan S, Skaar JR, Kuchay S, et al. mTOR generates an auto-amplification loop by triggering the bTrCP- and CK1a-dependent degradation of DEPTOR. Mol. Cell 2011; 44:317-24.
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