AHA1 Antibody
AHA1 Antibody, Clone 25F2.D10
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, ICC/IF, IP, E |
---|---|
Primary Accession | Q8BK64 |
Other Accession | NP_666148.1 |
Host | Rat |
Isotype | IgG2a Kappa |
Reactivity | Human, Mouse, Rat |
Clonality | Monoclonal |
Description | Rat Anti-Mouse AHA1 Monoclonal IgG2a Kappa |
Target/Specificity | Detects ~38kDa. Can run up to 45kDa on SDS Page. |
Other Names | Aha 1 Antibody, Ahsa1 Antibody, p38 Antibody, HSPC322 Antibody, C14orf3 Antibody, Activator of HSP90 ATPase Antibody, Activator of 90 kDa heat shock protein ATPase homolog 1 Antibody |
Clone Names | 25F2.D10 |
Immunogen | Mouse Aha1 |
Purification | Protein G Purified |
Storage | -20ºC |
Storage Buffer | 0.02M potassium phosphate, 0.15M sodium chloride, pH7.2 |
Shipping Temperature | Blue Ice or 4ºC |
Certificate of Analysis | 1 µg/ml of SMC-173 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody. |
Cellular Localization | Cytoplasm | Endoplasmic Reticulum |
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Provided below are standard protocols that you may find useful for product applications.
Background
Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
References
1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub.
2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796.
3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723.
4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
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