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TrpV3 Antibody

TRPV3 Antibody, Clone S15-39

     
  • IHC - TrpV3 Antibody ASM10210
    Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (ASM10210). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (ASM10210) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining in upper layers. Dull lower layer cell staining. Some stain seen in hypodermis.
    detail
  • WB - TrpV3 Antibody ASM10210
    Western Blot analysis of Rat brain membrane lysate showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (ASM10210). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (ASM10210) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
    detail
  • ICC/IF - TrpV3 Antibody ASM10210
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (ASM10210). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (ASM10210) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining in all cells. Some intermediate filament-like staining in some cells.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, IP, AM
Primary Accession Q4QYD9
Other Accession NP_001020928
Host Mouse
Isotype IgG2a
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat TrpV3 Monoclonal IgG2a
Target/Specificity Detects ~70kDa.
Other Names VRL3 Antibody, vanilloid receptor 2 Antibody, transient receptor potential cation channel subfamily V member 3 Antibody, sensitive channel TRPV3 Antibody, 1110036I10Rik Antibody, MGC124324 Antibody, MGC124325 Antibody, Trpv3 heat sensitive channel Antibody, vanilloid receptor-related osmotically activated channel protein Antibody
Clone Names S15-39
Immunogen Synthetic peptide amino acids 458-474 (cytoplasmic C-terminus) of rat TrpV3
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-334 was sufficient for detection of TrpV3 in 10 µg of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody
Cellular Localization Membrane
Citations (0)
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Background

The TRPV3 protein belongs to a family of non-selective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermo sensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. The gene lies in close proximity to another family member (TRPV1) gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels (1, 2).

References

1. Masamoto Y., Kawabata F., Fushiki T. (2009) Biosci. Biotechnol. Biochem. 73(5): 1021-1027.
2. Xiao R., et al. (2008) J Biol Chem. 283(10): 6162-6174.

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Cat# ASM10210
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