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mGluR1/5 Antibody

mGluR1/5 Antibody, Clone S75-33

     
  • ICC/IF - mGluR1/5 Antibody ASM10241
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 Monoclonal Antibody, Clone S75-33 (ASM10241). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-mGluR1/5 Monoclonal Antibody (ASM10241) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X.
    detail
  • WB - mGluR1/5 Antibody ASM10241
    Western Blot analysis of Rat brain membrane lysate showing detection of mGluR5 Glutamate Receptor protein using Mouse Anti-mGluR5 Glutamate Receptor Monoclonal Antibody, Clone S75-33 (ASM10241). Primary Antibody: Mouse Anti-mGluR5 Glutamate Receptor Monoclonal Antibody (ASM10241) at 1:1000.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, IP
Primary Accession P31424
Other Accession NP_058708.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat mGluR1/5 Monoclonal IgG1
Target/Specificity Detects ~130kDa (both mGluR1 and mGluR5).
Other Names Glutamate receptor metabotropic 5, GPRC1E Antibody, GRM 5 Antibody, GRM5 Antibody, Metabotropic glutamate receptor 5 Antibody, Metabotropic glutamate receptor 5 variant F Antibody, Metabotropic glutamate receptor 5 variant G Antibody, Metabotropic glutamate receptor 5 variant H Antibody, mGlu5 Antibody, mGluR5 Antibody, mGluR5a Antibody, mGluR5b Antibody, Glutamate receptor metabotropic 1 Antibody, glutamate receptor Antibody, metabotropic 1 Antibody, GPRC1A Antibody, GRM 1 Antibody, GRM1 alpha Antibody, GRM1 Antibody, GRM1_HUMAN Antibody, GRM1A Antibody, Metabotropic glutamate receptor 1 Antibody, mGlu1 Antibody, mGluR1 alpha Antibody, mGluR1 Antibody, mGluR1A Antibody
Clone Names S75-33
Immunogen Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-407 was sufficient for detection of mGluR1/5 glutamate receptor in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane
Research Areas
Citations (0)
citation

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Background

The AMPA receptor is a non-NMDA-type ionotropic transmembrane receptor for glutamate that mediates fast synaptic transmission in the CNS. AMPARs are composed of four types of subunits, designated as GluR1, GluR2, GluR3 and GluR4, which combine to form tetramers (1,2).

References

1. Mayer M.L. (2005) Current Opinion in Neurobio. 15(3): 282-288.
2. Keinane K., et al. (1990) Science. 249: 556-560.

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Cat# ASM10241
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