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ATG2A Antibody

     
  • ICC/IF - ATG2A Antibody  ASM10527
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG2A Polyclonal Antibody (ASM10527). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody (ASM10527) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG2A Antibody (D) Composite.
  • WB - ATG2A Antibody  ASM10527
    Western blot analysis of Mouse Brain showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody (ASM10527). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody (ASM10527) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa.
  • WB - ATG2A Antibody  ASM10527
    Western blot analysis of Human HeLa cell lysates showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody (ASM10527). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody (ASM10527) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q2TAZ0
Other Accession NP_055919.2
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human ATG2A Polyclonal
Target/Specificity Predicted molecular weights at ~213kDa. Other observed bands around ~90-100kDa to be expected.
Other Names ATG2 autophagy related homolog A Antibody, ATG2a_HUMAN Antibody, BC023754 Antibody, KIAA0404 Antibody, 1810013C15Rik Antibody
Immunogen Synthetic peptide from the C-terminal of human ATG2A
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-652 was sufficient for detection of ATG2A on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Preautophagosomal Structure Membrane | Peripheral membrane protein
Research Areas
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Background

ATG2A belongs to the ATG2 family. It is required for both autophagosome formation and regulation of lipid droplet morphology and dispersion (1).

References

1. Velikkakath A.K., Nishimura T., Oita E., Ishihara N., Mizushima N. (2012) Mol Biol Cell. 23(5): 896-909.

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Cat# ASM10527
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