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>   home   >   Products   >   Secondary Antibodies   >   Peroxidase (Horseradish)   >   Anti-Goat IgG (H&L) (Peroxidase Conjugated) Pre-Adsorbed Secondary Antibody   

Anti-Goat IgG (H&L) (Peroxidase Conjugated) Pre-Adsorbed Secondary Antibody

Rabbit Polyclonal, Peroxidase (Horseradish)

     
  • WB - Anti-Goat IgG (H&L)  (Peroxidase Conjugated) Pre-Adsorbed Secondary Antibody ASR2279
    Western Blot of Peroxidase conjugated Rabbit anti-Goat IgG antibody. Lane 1: Goat IgG. Lane 2: Human IgG. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:5,000 for 60 min at RT. Block: 5% BSA-TBS overnight at 4°C. Predicted/Observed size: 55 kDa, 28 kDa for Goat IgG. Other band(s): none.
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Product Information
Description Anti-GOAT IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated (Min X Human Serum Proteins)
Host Rabbit
Conjugate Peroxidase (Horseradish)
Target Species Goat
Reactivity Goat
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E, IC
Application Note ELISA 1:10,000-1:50,000
Western Blot 1:1,000-1:5,000
Immunochemistry 1:500-1:2,500
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Goat IgG (h&L) was produced by repeated immunization with goat IgG whole molecule in rabbit.
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
Citations (0)
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Background

Anti-Goat IgG (H&L) peroxidase conjugated antibody generated in rabbit detects specifically goat IgG. This secondary peroxidase conjugated antibody anti-goat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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$ 172.00
Cat# ASR2279
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Availability: 3-5 days
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