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USP33 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant USP33.

     
  • IF - USP33 Antibody (monoclonal) (M01) AT4487a
    Immunofluorescence of monoclonal antibody to USP33 on HeLa cell. [antibody concentration 20 ug/ml]
    detail
  • IP - USP33 Antibody (monoclonal) (M01) AT4487a
    Immunoprecipitation of USP33 transfected lysate using anti-USP33 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with USP33 MaxPab rabbit polyclonal antibody.
    detail
  • WB - USP33 Antibody (monoclonal) (M01) AT4487a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - USP33 Antibody (monoclonal) (M01) AT4487a
    Western Blot analysis of USP33 expression in transfected 293T cell line by USP33 monoclonal antibody (M01), clone 5B5.

    Lane 1: USP33 transfected lysate(103.3 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - USP33 Antibody (monoclonal) (M01) AT4487a
    Immunoperoxidase of monoclonal antibody to USP33 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
    detail
  • E - USP33 Antibody (monoclonal) (M01) AT4487a
    Detection limit for recombinant GST tagged USP33 is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, IP, E
Primary Accession Q8TEY7
Other Accession NM_015017
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 5B5
Calculated MW 106727 Da
Additional Information
Gene ID 23032
Other Names Ubiquitin carboxyl-terminal hydrolase 33, Deubiquitinating enzyme 33, Ubiquitin thioesterase 33, Ubiquitin-specific-processing protease 33, VHL-interacting deubiquitinating enzyme 1, hVDU1, USP33, KIAA1097, VDU1
Target/Specificity USP33 (NP_055832, 327 a.a. ~ 426 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsUSP33 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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References

Meta-analyses of genes modulating intracellular T3 bio-availability reveal a possible role for the DIO3 gene in osteoarthritis susceptibility. Meulenbelt I, et al. Ann Rheum Dis, 2010 Aug 19. PMID 20724312.Deubiquitinating enzyme USP33/VDU1 is required for Slit signaling in inhibiting breast cancer cell migration. Yuasa-Kawada J, et al. Proc Natl Acad Sci U S A, 2009 Aug 25. PMID 19706539.Midline crossing and Slit responsiveness of commissural axons require USP33. Yuasa-Kawada J, et al. Nat Neurosci, 2009 Sep. PMID 19684588.Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.The deubiquitinases USP33 and USP20 coordinate beta2 adrenergic receptor recycling and resensitization. Berthouze M, et al. EMBO J, 2009 Jun 17. PMID 19424180.

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$ 350.00
Cat# AT4487a
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