Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
APC Anti-Mouse IL-4 (11B11) Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC |
|---|---|
| Isotype | Rat IgG1, kappa |
| Concentration | 0.2 mg/mL |
| Reactivity | Mouse |
| Formulation | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2 |
| Host | Rat |
| Gene ID | 16189 |
|---|---|
| Gene Name | Il4 |
| Alternative Name(s) | IL4, Interleukin-4, BSF-1 |
| Format | APC |
| Preparation | This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze. |
| Application Notes | This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. |
| Storage Conditions | 2-8°C protected from light |
Cook PC, Jones LH, Jenkins SJ, Wynn TA, Allen JE, and MacDonald AS. 2012. Proc. Natl. Acad. Sci. 109: 9977-9982. (in vivo blocking)
Altin JA, Goodnow CC, and Cook MC. 2012. J. Immunol. 5478-5488. (Flow cytometry)
Tofukuji S, Kuwahara M, Suzuki J, Ohara O, Nakayama T, and Yamashita M. 2012. J. Immunol. 188: 4846-4857. (in vitro Th1 polarization)
Weber KS, Hildner K, Murphy KM and Allen PM. 2010 J. Immunol. 185: 2836-2846 (in vitro Th1 polarization, ELISA)
Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, and Holdsworth SR. 2005. J. Am. Soc. Nephrol. 16: 2012-2022. (in vivo activation, Immunofluorescence microscopy – frozen tissue, Immunohistochemistry – frozen tissue)
Provided below are standard protocols that you may find useful for product applications.
Background
The 11B11 antibody binds to mouse Interleukin-4 (IL-4), a 14 kDa cytokine that is largely secreted by activated T cells of the Th2 subset, and to some degree by NKT and mast cells. This cytokine acts as a stimulatory factor for B cells, inducing their proliferation and differentiation, as well as playing a role in immunoglobulin class-switching. IL-4 may also provide autocrine stimulation for T cells, and affect the function of antigen presenting cells such as macrophages and dendritic cells. IL-4 can bind and signal via three cell surface receptor types: CD124 by itself, CD124 in combination with the common gamma chain (type I complex), or CD124 combined with CD213a1 (type II complex).
The 11B11 antibody is widely used for detection of intracellular levels of IL-4 protein by flow cytometry, as well as for analysis of soluble cytokine as measured by ELISA, and in functional assays to neutralization cytokine-receptor interactions.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
