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PACSIN2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - PACSIN2 Antibody (C-term) AW5026-U100
    Immunofluorescent analysis of Hela cells, using PACSIN2 Antibody (C-term) (Cat. #AW5026). AW5026 was diluted at 1:100 dilution. Alexa Fluor® 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
    detail
  • WB - PACSIN2 Antibody (C-term) AW5026-U100
    PACSIN2 Antibody (S357) (Cat.# AW5026) western blot analysis in Daudi cell line and mouse brain lysates (35ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).
    detail
  • WB - PACSIN2 Antibody (C-term) AW5026-U100
    Western blot analysis of lysates from rat heart tissue lysate and Daudi cell line (from left to right), using PACSIN2 Antibody (S357)(Cat. #AW5026). AW5026 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
    detail
  • IHC-P - PACSIN2 Antibody (C-term) AW5026-U100
    Immunohistochemical analysis of paraffin-embedded H. stomach section using PACSIN2 Antibody (C-term)(Cat#AW5026). AW5026 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • IHC-P - PACSIN2 Antibody (C-term) AW5026-U100
    Immunohistochemical analysis of paraffin-embedded H.stomach section using PACSIN2 Antibody (C-term)(Cat#AW5026). AW5026 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, IF
Primary Accession Q9UNF0
Reactivity Human, Rat
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=56;M=56;Rat=56 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 11252
Antigen Region 342-371 aa
Other Names PACSIN2; Protein kinase C and casein kinase substrate in neurons protein 2
Dilution IF~~1:100
WB~~1:1000
IHC-P~~1:100
Target/Specificity This PACSIN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 amino acids from the C-terminal region of human PACSIN2.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPACSIN2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PACSIN2
Function Regulates the morphogenesis and endocytosis of caveolae (By similarity). Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome. Recycling endosome membrane Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection Membrane, caveola. Note=Detected at the neck of flask-shaped caveolae. Localization to tubular recycling endosomes probably requires interaction with MICALL1 and EHD1
Tissue Location Widely expressed.
Research Areas
Citations (0)
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Background

PACSIN may play a role in vesicle formation and transport. This protein homo- and hetero-aggregates with other PACSINs. It also binds dynamin 1, synaptojanin, synapsin 1 and the neural Wiskott-Aldrich syndrome protein (N-WASP). The protein exhibits a cvesicle-like cytoplasmic distribution and is ubiquitously expressed. PACSIN is phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). The protein contains 1 FCH domain and 1 SH3 domain.

References

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Wiemann, S., et al., Genome Res. 11(3):422-435 (2001). Ritter, B., et al., FEBS Lett. 454(3):356-362 (1999). Dunham, I., et al., Nature 402(6761):489-495 (1999).

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$ 370.00
Cat# AW5026-U100
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