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ACHE Antibody (C-term)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • IHC - ACHE Antibody (C-term) AW5506-U100
    Immunohistochemical analysis of paraffin-embedded Human brain section using Pink1(Cat#AW5506). AW5506 was diluted at 1:500 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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  • WB - ACHE Antibody (C-term) AW5506-U100
    All lanes : Anti-ACHE Antibody (C-term) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lane 3: COS-7 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: mouse cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - ACHE Antibody (C-term) AW5506-U100
    All lanes : Anti-ACHE Antibody (C-term) at 1:2000 dilution Lane 1: COS-7 whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: mouse cerebellum lysates Lane 4: NIH/3T3 whole cell lysates Lane 5: rat cerebellum lysates Lane 6: Raji whole cell lysates Lane 7: CHO whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P22303
Reactivity Human, Mouse, Rat, Hamster
Host Mouse
Clonality Monoclonal
Calculated MW H=68;M=68 KDa
Isotype IgG1
Antigen Source HUMAN
Additional Information
Gene ID 43
Antigen Region 597-631 aa
Other Names Acetylcholinesterase, AChE, ACHE
Dilution IHC~~1:500
WB~~1:2000
Target/Specificity This ACHE antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 597-631 amino acids from the C-terminal region of human ACHE.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACHE Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACHE (HGNC:108)
Function Hydrolyzes rapidly the acetylcholine neurotransmitter released into the synaptic cleft allowing to terminate the signal transduction at the neuromuscular junction. Role in neuronal apoptosis.
Cellular Location Synapse. Secreted. Cell membrane; Peripheral membrane protein [Isoform H]: Cell membrane; Lipid- anchor, GPI-anchor; Extracellular side
Tissue Location Isoform H is highly expressed in erythrocytes.
Research Areas
Citations (0)
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Background

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.

References

Soreq H.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:9688-9692(1990).
Karpel R.,et al.Exp. Cell Res. 210:268-277(1994).
Yang L.,et al.Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Totoki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.

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$ 380.00
Cat# AW5506-U100
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