MCP-1 (MCAF), human recombinant protein
MCP1, SCYA2
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P10148 |
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Calculated MW | 8.6 kDa |
Gene ID | 20296 |
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Gene Symbol | MCP-1 |
Other Names | HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte chemotactic protein 1, MCP-1, Monocyte secretory protein JE, Small-inducible cytokine A2 |
Gene Source | Mouse |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥98% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Results | 4 - 15 ng/ml. |
Sequence | QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN |
Target/Specificity | MCP-1/CCL2 |
Application Notes | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
Format | Lyophilized protein |
Storage | -20°C; Sterile filtered and lyophilized with no additives |
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Provided below are standard protocols that you may find useful for product applications.
Background
Monocyte Chemoattractant Protein 1 (MCP-1), also known as CCL2, is thought to be produced by injured or infected tissues. MCP-1 signals through G protein-coupled receptors, CCR2 and CCR4, to recruit memory T cells, monocytes and dendritic cells. Recombinant mouse MCP-1 is a non-glycosylated protein, containing 76 amino acids and having a molecular mass of 8.6 kDa.
References
Kawahara R.S.,et al.J. Biol. Chem. 264:679-682(1989).
Rollins B.J.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:3738-3742(1988).
Teuscher C.,et al.J. Immunol. 163:2262-2266(1999).
van Damme J.,et al.Eur. J. Biochem. 199:223-229(1991).
Ueno M.,et al.J. Lipid Res. 54:734-743(2013).
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