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Anti-Eotaxin Antibody

     
  • WB - Anti-Eotaxin Antibody ABO10845
    Anti- Eotaxin antibody, ABO10845, Western blottingAll lanes: Anti Eotaxin(ABO10845) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 11KDObserved bind size: 11KD
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P51671
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Eotaxin(CCL11) detection. Tested with WB in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 6356
Other Names Eotaxin, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11, CCL11, SCYA11
Calculated MW 10732 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Secreted.
Protein Name Eotaxin
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Eotaxin(73-92aa CADPKKKWVQDSMKYLDQKS).
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the intercrine beta (chemokine CC) family.
Protein Information
Name CCL11
Synonyms SCYA11
Function In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions (PubMed:8597956). Binds to CCR3 (PubMed:8631813).
Cellular Location Secreted.
Research Areas
Citations (0)
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Background

Eotaxin, also known as CCL11(Chemokine(C-C motif) ligand 11), a small cytokine belonging to the CC chemokine family. Eotaxin selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses.The effects of Eotaxin are mediated by its binding to a G-protein-linked receptor known as a chemokine receptor. Chemokine receptors for which Eotaxinis a ligand include CCR2, CCR3 and CCR5. However, it has been found that eotaxin-1(CCL11) has high degree selectivity for its receptor, such that they are inactive on neutrophils and monocytes, which do not express CCR3. The gene for human Eotaxin is encoded on three exons and is located on chromosome 17q12.

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$ 240.00
Cat# ABO10845
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