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TSC2 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P49816 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 201278 Da |
| Gene ID | 24855 |
|---|---|
| Positive Control | Jurkat (Induced and Uninduced), Rat kidney, 3T3 cell lysates |
| Application & Usage | The antibody can be used for Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately. |
| Other Names | anti-TSC2, Tuberin, Tuberous sclerosis 2 protein homolog |
| Target/Specificity | TSC2 |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 200 µg (0.5 mg/ml) affinity purified rabbit anti-TSC2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | TSC2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Tsc2 {ECO:0000303|PubMed:8519695, ECO:0000312|RGD:3908} |
|---|---|
| Function | Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed:12172553). Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1 (By similarity). In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling (By similarity). The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1 (By similarity). Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling (PubMed:16707451). Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (PubMed:9045618). |
| Cellular Location | Lysosome membrane {ECO:0000250|UniProtKB:P49815}; Peripheral membrane protein {ECO:0000250|UniProtKB:P49815}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:P49815}. Note=Recruited to lysosomal membranes in a RHEB-dependent process in absence of nutrients (By similarity). In response to insulin signaling and phosphorylation by PKB/AKT1, the complex dissociates from lysosomal membranes and relocalizes to the cytosol (By similarity) {ECO:0000250|UniProtKB:P49815} |
| Tissue Location | CNS, uterus, heart, skeletal muscle, kidney and spleen. |
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Provided below are standard protocols that you may find useful for product applications.
Background
TSC2 is important in regulating cell proliferation and tumor development. Mutations in either the TSC1 or TSC2 tumor suppressor genes caused tuberous sclerosis complex (TSC), an autosomal dominant tumor syndrome. Tuberin is a product of the TSC2 tumor suppressor gene. Tuberin form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR).
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