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ApoA1 Antibody

Rabbit Polyclonal Antibody

     
  • WB - ApoA1 Antibody ABV11049-30
    Western blot analysis using recombinant human ApoA1. Lane 1: 250 ng;Lane 2: 50 ng;Lane 3: 10 ng
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P04639
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 30062 Da
Additional Information
Gene ID 25081
Application & Usage Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Recombinant ApoA1 can be used as a positive control. The antibody recognizes ~28 kDa human ApoA1. Reactivity to other species has not been tested.
Other Names Apolipoprotein A-1, Apo-A1, ApoA-1,
Target/Specificity ApoA1
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit anti-rat A1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol and 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsApoA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Apoa1
Function Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Cellular Location Secreted.
Tissue Location Major protein of plasma HDL, also found in chylomicrons
Citations (0)
citation

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Background

Apolipoprotein A-I (ApoA1) is the primary protein constituent of HDL, defining its size and shape, solubilizing its lipid components, removing cholesterol from peripheral cells, activating the LCAT enzyme, and delivering the resulting cholesterol esters to the liver.

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Discontinued
Cat# ABV11049-30
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