Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
EHHADH Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q08426 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 79495 Da |
| Gene ID | 1962 |
|---|---|
| Positive Control | Western Blot: Jurkat cell lysate, 3T3 cell lysate |
| Application & Usage | Western blot: 1-4 µg |
| Other Names | Enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase, LBFP, LBP, L-PBE, peroxisomal bifunctional enzyme3, 2-trans-enoyl-CoA isomerase, peroxisomal enoyl-CoA hydratase |
| Target/Specificity | EHHADH |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.5 mg/ml) of Caspase-10 antibody in PBS pH 7.2, 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | EHHADH Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | EHHADH (HGNC:3247) |
|---|---|
| Synonyms | ECHD |
| Function | Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl- CoA isomerase activities. Catalyzes two of the four reactions of the long chain fatty acids peroxisomal beta-oxidation pathway (By similarity). Can also use branched-chain fatty acids such as 2-methyl- 2E-butenoyl-CoA as a substrate, which is hydrated into (2S,3S)-3- hydroxy-2-methylbutanoyl-CoA (By similarity). Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species (Probable). Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species (By similarity). With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity (PubMed:15060085). Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways (By similarity). Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta- oxidation (PubMed:15060085). |
| Cellular Location | Peroxisome. |
| Tissue Location | Liver and kidney. Strongly expressed in the terminal segments of the proximal tubule. Lower amounts seen in the brain. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
EHHADH is a bifunctional enzyme. It is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of this protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. EHHADH-null mice only exhibit a blunted peroxisome proliferative response when challenged with a peroxisome proliferator.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
