Anti-ZNRF2 Antibody
Rabbit Polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, IF |
---|---|
Primary Accession | Q8NHG8 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 24115 Da |
Gene ID | 223082 |
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Positive Control | WB: HEK293T, A549, RAW264.7, H9C2 cel lysate; IHC: human brain tissue; IFC: A549 cells |
Application & Usage | WB; 1:500 – 1:2000, IHC; 1:50 – 1:200, IF/IC; 1:50 – 1:100 |
Alias Symbol | ZNRF2 |
Other Names | RNF202, E3 ubiquitin-protein ligase ZNRF2, Protein Ells2, RING finger protein 202, Zinc/RING finger protein 2 |
Formulation | In 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol and 0.01% sodium azide |
Reconstitution & Storage | 12 months under -20°C |
Background Descriptions | |
Precautions | Anti-ZNRF2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ZNRF2 |
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Synonyms | RNF202 |
Function | E3 ubiquitin-protein ligase that plays a role in the establishment and maintenance of neuronal transmission and plasticity. Ubiquitinates the Na(+)/K(+) ATPase alpha-1 subunit/ATP1A1 and thereby influences its endocytosis and/or degradation (PubMed:22797923). Acts also as a positive regulator of mTORC1 activation by amino acids, which functions upstream of the V-ATPase and of Rag-GTPases (PubMed:27244671). In turn, phosphorylation by mTOR leads to its inhibition via targeting to the cytosol allowing a self-regulating feedback mechanism (PubMed:27244671). |
Cellular Location | Endosome membrane; Peripheral membrane protein. Lysosome membrane; Peripheral membrane protein. Presynaptic cell membrane; Peripheral membrane protein. Cytoplasm |
Tissue Location | Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney. |
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Provided below are standard protocols that you may find useful for product applications.
Background
E3 ubiquitin-protein ligase ZNRF2 protein plays a role in the establishment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
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