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AKAP12 Antibody (C-Term)

Peptide-affinity purified goat antibody

     
  • WB - AKAP12 Antibody (C-Term) AF2237a
    AF2237a(0.02 µg/ml) staining of Human Uterus lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q02952
Other Accession NP_005091.2, NP_653080.1, 9590
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 191482 Da
Additional Information
Gene ID 9590
Other Names A-kinase anchor protein 12, AKAP-12, A-kinase anchor protein 250 kDa, AKAP 250, Gravin, Myasthenia gravis autoantigen, AKAP12, AKAP250
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAKAP12 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AKAP12
Synonyms AKAP250
Function Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
Cellular Location Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Membrane; Lipid-anchor. Note=May be part of the cortical cytoskeleton
Tissue Location Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells
Research Areas
Citations (0)
citation

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Background

This anrtibody is expected to recognize both reported isoforms.

References

Gravin, an autoantigen recognized by serum from myasthenia gravis patients, is a kinase scaffold protein. Nauert JB, Klauck TM, Langeberg LK, Scott JD. Curr Biol. 1997 Jan 1;7(1):52-62. PMID: 9000000

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Discontinued
Cat# AF2237a
Size:
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