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EVL Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - EVL Antibody (internal region) AF2895a
    AF2895a (1 µg/ml) staining of Jurkat lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9UI08
Other Accession NP_057421.1, 51466, 14026 (mouse), 79115 (rat)
Reactivity Human
Predicted Mouse, Rat
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 44620 Da
Additional Information
Gene ID 51466
Other Names Ena/VASP-like protein, Ena/vasodilator-stimulated phosphoprotein-like, EVL, RNB6
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEVL Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EVL
Synonyms RNB6
Function Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.
Cellular Location Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P70429}. Cytoplasm, cytoskeleton, stress fiber {ECO:0000250|UniProtKB:P70429}. Cell projection, lamellipodium {ECO:0000250|UniProtKB:P70429}. Note=Targeted to the leading edge of lamellipodia and the distal tip of stress fibers through interaction with a number of proteins. In activated T-cells, localizes to the F- actin collar and the distal tip of microspikes {ECO:0000250|UniProtKB:P70429}
Research Areas
Citations (0)
citation

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References

Filopodia are required for cortical neurite initiation. Dent EW, Kwiatkowski AV, Mebane LM, Philippar U, Barzik M, Rubinson DA, Gupton S, Van Veen JE, Furman C, Zhang J, Alberts AS, Mori S, Gertler FB. Nat Cell Biol. 2007 Dec;9(12):1347-59. Epub 2007 Nov 18. PMID: 18026093

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$ 341.00
Cat# AF2895a
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Availability: 7-10 days
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