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GLI3 Antibody (aa41-57)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, ICC, E |
|---|---|
| Primary Accession | P10071 |
| Reactivity | Human, Chicken, Quail, Xenopus, Dog |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 170kDa |
| Dilution | ELISA (1:6000-1:30000), IHC-P (1.25 µg/ml), WB (1:500-1:2000) |
| Gene ID | 2737 |
|---|---|
| Other Names | Transcriptional activator GLI3, GLI3 form of 190 kDa, GLI3-190, GLI3 full length protein, GLI3FL, Transcriptional repressor GLI3R, GLI3 C-terminally truncated form, GLI3 form of 83 kDa, GLI3-83, GLI3 |
| Target/Specificity | Amino acids 41-57 of human Gli-3 protein. |
| Reconstitution & Storage | Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles. |
| Precautions | GLI3 Antibody (aa41-57) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GLI3 |
|---|---|
| Function | Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI- consensus sequence 5'-GGGTGGTC-3'. |
| Cellular Location | Nucleus. Cytoplasm. Cell projection, cilium. Note=GLI3FL is localized predominantly in the cytoplasm while GLI3R resides mainly in the nucleus. Ciliary accumulation requires the presence of KIF7 and SMO. Translocation to the nucleus is promoted by interaction with ZIC1 |
| Tissue Location | Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
References
Ruppert J.M.,et al.Mol. Cell. Biol. 10:5408-5415(1990).
Kalff-Suske M.,et al.Hum. Mol. Genet. 8:1769-1777(1999).
Hillier L.W.,et al.Nature 424:157-164(2003).
Scherer S.W.,et al.Science 300:767-772(2003).
Ruppert J.M.,et al.Mol. Cell. Biol. 8:3104-3113(1988).
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