FEM1B Antibody (aa609-622)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF |
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Primary Accession | Q9UK73 |
Reactivity | Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Horse, Bovine, Dog |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 70kDa |
Dilution | IHC-P (10 µg/ml), WB (0.5-1 µg/ml), |
Gene ID | 10116 |
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Other Names | Protein fem-1 homolog B, FEM1b, FEM1-beta, Fem-1-like death receptor-binding protein alpha, Fem-1-like in apoptotic pathway protein alpha, F1A-alpha, FEM1B, F1AA, KIAA0396 |
Target/Specificity | Peptide (DINYQDQIPRTLEE) corresponding to amino acids 609 to 622 of human F1Aa This sequence is identical to the corresponding sequence of mouse FEM1b. |
Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted. |
Precautions | FEM1B Antibody (aa609-622) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | FEM1B {ECO:0000303|PubMed:10623617, ECO:0000312|HGNC:HGNC:3649} |
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Function | Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed:29779948, PubMed:33398170, PubMed:33398168). The C- degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms (PubMed:29779948, PubMed:33398170, PubMed:33398168). The CRL2(FEM1B) complex specifically recognizes proteins ending with -Gly-Leu-Asp-Arg, such as CDK5R1, leading to their ubiquitination and degradation (PubMed:33398170, PubMed:33398168). Also acts as a regulator of the reductive stress response by mediating ubiquitination of reduced FNIP1: in response to reductive stress, the CRL2(FEM1B) complex specifically recognizes a conserved Cys degron in FNIP1 when this degron is reduced, leading to FNIP1 degradation and subsequent activation of mitochondria to recalibrate reactive oxygen species (ROS) (By similarity). Mechanistically, recognizes and binds reduced FNIP1 through two interface zinc ions, which act as a molecular glue that recruit reduced FNIP1 to FEM1B (By similarity). Promotes ubiquitination of GLI1, suppressing GLI1 transcriptional activator activity (PubMed:24076122). Promotes ubiquitination and degradation of ANKRD37 (By similarity). Promotes ubiquitination and degradation of SLBP (PubMed:28118078). Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis (PubMed:10542291). Also involved in glucose homeostasis in pancreatic islet (By similarity). May also act as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1 (PubMed:19330022). |
Cellular Location | Cytoplasm. Nucleus Note=In the nucleus, the protein level increased slightly after camptothecin (CPT) treatment (PubMed:19330022). Associated with chromatin (PubMed:19330022). |
Tissue Location | Widely expressed (PubMed:10542291). Highly expressed in testis (PubMed:10542291). Weakly expressed in other tissues (PubMed:10542291). |
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Background
Component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. Functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1.
References
Chan S.-L.,et al.J. Biol. Chem. 274:32461-32468(1999).
Ventura-Holman T.,et al.Biochem. Biophys. Res. Commun. 267:317-320(2000).
Ishikawa K.,et al.DNA Res. 4:307-313(1997).
Nakajima D.,et al.DNA Res. 9:99-106(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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