STT3A / ITM1 Antibody (clone 4D4)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | P46977 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 4D4 |
Calculated MW | 81kDa |
Dilution | IHC-P (5 µg/ml) |
Gene ID | 3703 |
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Other Names | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A, Oligosaccharyl transferase subunit STT3A, STT3-A, 2.4.99.18, B5, Integral membrane protein 1, Transmembrane protein TMC, STT3A, ITM1, TMC |
Reconstitution & Storage | Store at -20°C. Aliquot to avoid freeze/thaw cycles. |
Precautions | STT3A / ITM1 Antibody (clone 4D4) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | STT3A (HGNC:6172) |
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Synonyms | ITM1, TMC |
Function | Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol- pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667, PubMed:34653363). N- glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid- linked oligosaccharide (LLO) binding pockets (By similarity). STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B- containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A (PubMed:19167329). |
Cellular Location | Endoplasmic reticulum. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P46978}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P46978} |
Tissue Location | Expressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney Expressed in skin fibroblasts (at protein level) |
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Background
Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient cotranslational glycosylation and mediate glycosylation of sites that have been skipped by STT3A.
References
Hong G.,et al.Genomics 31:295-300(1996).
Lissy N.A.,et al.Biochim. Biophys. Acta 1306:137-141(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Taylor T.D.,et al.Nature 440:497-500(2006).
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