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DDH / AKR1C1 Antibody (clone AT6D10)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q04828 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | AT6D10 |
| Calculated MW | 37kDa |
| Dilution | IHC-P (10 µg/ml), WB (1:1000), |
| Gene ID | 1645 |
|---|---|
| Other Names | Aldo-keto reductase family 1 member C1, 1.1.1.-, 20-alpha-hydroxysteroid dehydrogenase, 20-alpha-HSD, 1.1.1.149, Chlordecone reductase homolog HAKRC, Dihydrodiol dehydrogenase 1/2, DD1/DD2, High-affinity hepatic bile acid-binding protein, HBAB, Indanol dehydrogenase, 1.1.1.112, Trans-1, 2-dihydrobenzene-1, 2-diol dehydrogenase, 1.3.1.20, AKR1C1, DDH, DDH1 |
| Target/Specificity | Human AKR1C1 |
| Reconstitution & Storage | Can be stored at 4°C. For long term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing cycles. |
| Precautions | DDH / AKR1C1 Antibody (clone AT6D10) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | AKR1C1 |
|---|---|
| Synonyms | DDH, DDH1 |
| Function | Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids (PubMed:19218247). Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH (PubMed:14672942). Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens (PubMed:10998348). May also reduce conjugated steroids such as 5alpha- dihydrotestosterone sulfate (PubMed:19218247). Displays affinity for bile acids (PubMed:8486699). |
| Cellular Location | Cytoplasm, cytosol. |
| Tissue Location | Expressed in all tissues tested including liver, prostate, testis, adrenal gland, brain, uterus, mammary gland and keratinocytes. Highest levels found in liver, mammary gland and brain |
| Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Converts progesterone to its inactive form, 20-alpha- dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation.
References
Stolz A.,et al.J. Biol. Chem. 268:10448-10457(1993).
Lou H.,et al.J. Biol. Chem. 269:8416-8422(1994).
Ciaccio P.J.,et al.J. Biol. Chem. 269:15558-15562(1994).
Khanna M.,et al.J. Steroid Biochem. Mol. Biol. 53:41-46(1995).
Nishizawa M.,et al.Genes Cells 5:111-125(2000).
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