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SAE1 Antibody (clone 1G8)

Mouse Monoclonal Antibody

     
  • WB - SAE1 Antibody (clone 1G8) ALS15828
    HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAE1...
    detail
  • IF - SAE1 Antibody (clone 1G8) ALS15828
    Anti-SAE1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently...
    detail
  • IHC - SAE1 Antibody (clone 1G8) ALS15828
    Anti-SAE1 antibody IHC staining of human prostate.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession Q9UBE0
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1G8
Calculated MW 38kDa
Dilution IF (1:100), IHC-P (10 µg/ml), WB (1:4000)
Additional Information
Gene ID 10055
Other Names SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, SUMO-activating enzyme subunit 1, N-terminally processed, SAE1, AOS1, SUA1, UBLE1A
Target/Specificity Human SAE1
Reconstitution & Storage Store at -20°C. Minimize freezing and thawing.
PrecautionsSAE1 Antibody (clone 1G8) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SAE1
Synonyms AOS1, SUA1, UBLE1A
Function The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Cellular Location Nucleus.
Tissue Location Expression level increases during S phase and drops in G2 phase (at protein level).
Volume 50 µl
Research Areas
Citations (0)
citation

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Background

The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

References

Okuma T.,et al.Biochem. Biophys. Res. Commun. 254:693-698(1999).
Gong L.,et al.FEBS Lett. 448:185-189(1999).
Desterro J.M.P.,et al.J. Biol. Chem. 274:10618-10624(1999).
Zhang Q.-H.,et al.Genome Res. 10:1546-1560(2000).
Li W.B.,et al.Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.

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Discontinued
Cat# ALS15828
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