SAE1 Antibody (clone 1G8)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF |
---|---|
Primary Accession | Q9UBE0 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 1G8 |
Calculated MW | 38kDa |
Dilution | IF (1:100), IHC-P (10 µg/ml), WB (1:4000) |
Gene ID | 10055 |
---|---|
Other Names | SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, SUMO-activating enzyme subunit 1, N-terminally processed, SAE1, AOS1, SUA1, UBLE1A |
Target/Specificity | Human SAE1 |
Reconstitution & Storage | Store at -20°C. Minimize freezing and thawing. |
Precautions | SAE1 Antibody (clone 1G8) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SAE1 |
---|---|
Synonyms | AOS1, SUA1, UBLE1A |
Function | The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. |
Cellular Location | Nucleus. |
Tissue Location | Expression level increases during S phase and drops in G2 phase (at protein level). |
Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
References
Okuma T.,et al.Biochem. Biophys. Res. Commun. 254:693-698(1999).
Gong L.,et al.FEBS Lett. 448:185-189(1999).
Desterro J.M.P.,et al.J. Biol. Chem. 274:10618-10624(1999).
Zhang Q.-H.,et al.Genome Res. 10:1546-1560(2000).
Li W.B.,et al.Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
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