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UFD2A / UBE4B Antibody (aa869-1179)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, ICC |
|---|---|
| Primary Accession | O95155 |
| Other Accession | 10277 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 146185 Da |
| Dilution | ICC (1:100 - 1:1000), IHC-P (7.5 µg/ml), WB (1:5000 - 1:20000), |
| Gene ID | 10277 |
|---|---|
| Other Names | UBE4B, KIAA0684, HDNB1, Homologous to yeast UFD2, UFD2, UFD2A, Ubiquitination factor E4B, UBOX3, E4 |
| Target/Specificity | Human UFD2A / UBE4B |
| Reconstitution & Storage | 0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal. Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Precautions | UFD2A / UBE4B Antibody (aa869-1179) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | UBE4B (HGNC:12500) |
|---|---|
| Function | Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (By similarity). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (PubMed:17369820). |
| Cellular Location | Cytoplasm. Nucleus {ECO:0000250|UniProtKB:Q9ES00} |
| Tissue Location | Expressed in differentiated myotubes (at protein level) (PubMed:17369820). Highest expression in ovary, testis, heart and skeletal muscle (PubMed:11802788). Expression is low in colon, thymus and peripheral blood leukocytes (PubMed:11802788). Almost undetectable in lung and spleen (PubMed:11802788) |
| Volume | 50 µl |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase.
References
Onyango P.,et al.Genomics 50:187-198(1998).
Ohira M.,et al.Oncogene 19:4302-4307(2000).
Mahoney J.A.,et al.Biochem. J. 361:587-595(2002).
Mammen A.L.,et al.PLoS ONE 6:E28861-E28861(2011).
Ishikawa K.,et al.DNA Res. 5:169-176(1998).
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