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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   C4orf52 Polyclonal Antibody   

C4orf52 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - C4orf52 Polyclonal Antibody AP55863
    Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
    Incubation: Anti-C4orf52 Polyclonal Antibody, Unconjugated(bs-15196R) 1:5000, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • WB - C4orf52 Polyclonal Antibody AP55863
    Sample: muscle (Mouse) Lysate at 40 ug
    Primary: Anti-C4orf52(bs-15196R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 18 kD
    Observed band size: 18 kD
    detail
  • WB - C4orf52 Polyclonal Antibody AP55863
    Sample: heart (Mouse) Lysate at 40 ug
    Primary: Anti-C4orf52(bs-15196R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 18 kD
    Observed band size: 18 kD
    detail
  • IHC-P - C4orf52 Polyclonal Antibody AP55863
    Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C4orf52) Polyclonal Antibody, Unconjugated (bs-15196R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    detail
  • IHC-P - C4orf52 Polyclonal Antibody AP55863
    Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C4orf52) Polyclonal Antibody, Unconjugated (bs-15196R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    detail
  • IHC-P - C4orf52 Polyclonal Antibody AP55863
    Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C4orf52) Polyclonal Antibody, Unconjugated (bs-15196R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q8N5G0
Reactivity Rat, Dog, Cow
Host Rabbit
Clonality Polyclonal
Calculated MW 7702 Da
Additional Information
Gene ID 389203
Other Names Small integral membrane protein 20, Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 7 kDa, MITRAC7, Phoenixin-14, SMIM20, C4orf52, MITRAC7 {ECO:0000303|PubMed:26321642}
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name SMIM20
Synonyms C4orf52, MITRAC7 {ECO:0000303|PubMed:263
Function [Small integral membrane protein 20]: Component of the MITRAC (mitochondrial translation regulation assembly intermediate of cytochrome c oxidase complex) complex, that regulates cytochrome c oxidase assembly (PubMed:26321642). Promotes the progression of complex assembly after the association of MT-CO1/COX1 with COX4I1 and COX6C (PubMed:26321642). Chaperone-like assembly factor required to stabilize newly synthesized MT-CO1/COX1 and to prevent its premature turnover (PubMed:26321642).
Cellular Location [Small integral membrane protein 20]: Mitochondrion inner membrane; Single-pass membrane protein [Phoenixin-20]: Secreted {ECO:0000250|UniProtKB:C0HLM6}
Tissue Location Expressed in the ovary, specifically in granulosa cells of follicles that have passed the primary stage and in oocytes (at protein level).
Research Areas
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$ 350.00
Cat# AP55863
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